Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Starch (淀粉) 10g NaCl 5g
CaCO3 3g Agar (琼脂) 20g
Distilled water (蒸馏水) 1L pH 7.0
30. Wheat Bran Medium (麸皮培养基)
Wheat bran (麸皮) 36g (NH4)2HPO4 10g
K2HPO4 0.2g MgSO4。7H2O 0.1g
Agar (琼脂) 20g Distilled water (蒸馏水) 1L
pH 7.0
31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (琼脂) 2%
Trace element solution (混合盐类) 1ml/100ml
[Note]: Trace element solution (混合盐类溶液): MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (琼脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸钠) 0.82%
Agar (琼脂) 1.5%
32. BPY Medium (BPY 培养基)
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (琼脂) 1-2%
Distilled (蒸馏水) 1000ml pH 7.0
适用范围:大肠埃希氏菌(大肠杆菌)、恶臭假单胞菌
33. LB Medium (LB 培养基)
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
NaCl 10g Agar (琼脂) 1-2%
Distilled water (蒸馏水) 1000ml pH 7.0
适用范围:大肠埃希氏菌(大肠杆菌)
34. Penssay Broth (Penssay 肉汤)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白胨) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
Distilled water (蒸馏水) 1000ml pH 7.2
35. Blond Agar (血琼脂培养基)
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml
Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze . [注]猪心和猪脑浸汁法:(1)新鲜猪心除去心头和脂肪,搅碎100克加蒸馏水至1000毫升;(2)新鲜猪脑除去血管,取200克加蒸馏水至1000毫升;(3)50℃保温1小时,然后煮开15分钟;(4)冷却后用纱布过滤备用。
36. Cellulosedecomposing Bacteria Synthetic Medium (纤维细菌合成培养基)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
(NH4)2SO4 2.0g Distilled water (蒸馏水) 1000ml
Filter paper strip: one strip (滤纸条:一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNa )
37. Clostridium Pasreurianum Synthetic Medium (巴氏梭状芽孢杆菌合成培养基)
Glucose (葡萄糖) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸馏水) 100% pH 7.0
38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麦芽膏琼脂 Ⅱ)
Yeast extract (酵母膏) 4g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Agar (琼脂) 17g
Distilled water (蒸馏) 1000ml pH 7.3
39. Oatemeal Agar (燕麦粉琼脂)
Oatemeal(infusion)[燕麦粉浸液] 20g Agar (琼脂) 18%
Trace element solution (迹量盐溶液)1ml
Distilled water up to (用蒸馏水补充至1000毫升) 1000ml pH 7.2
[Note]:Trace element solution (迹量盐溶液):
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸馏水) 100ml
40. Calcium Malate Agar (苹果酸钙琼脂)
Calium malate (苹果酸钙) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml
41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (蒸馏水) 1000ml pH 2.0
42. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [无水] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (蒸馏水) 1000ml
pH 3.5-4
43. Plasma Substitute Medium (代血浆培养基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
44. Skim Milk Medium (脱脂牛奶培养基) ATCC 183
脱脂牛奶 100g 去离子水 1000ml
适用范围:保加利亚乳杆菌、嗜热乳酸链球菌
45. Pea Agar (豌豆琼脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (琼脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2计)] 12-14 mgM100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆900克,置于5公升广口瓶中,加自来水3公升,用硫酸调pH至4.3-4.5,再加氯仿30毫升(防腐),加胶塞并用纱布扎口(防止冲脱),放37℃恒温室7-10天。取出用滤纸过滤,滤液煮沸20分钟,以除氯仿,并再次以滤纸过滤,除沉淀。滤液分装,于120℃30分钟灭菌。后冷藏备用。)
The standard quality of pea infusion as following (豌豆浸汁质量标准):
Aminonitrogen(氨基酸): 160-200 mgM100ml
Soluble phosphoruse(溶磷): 400-600 μgMml
Color(色泽): auratus,transparent (金黄、透明)
46. Wheat Bran Medium (麦麸琼脂)
Wheat bran (麦麸) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (琼脂) 2% pH 7.0
适用范围:金霉素链霉菌、红霉素链霉菌
47. Glucose Yeast Extract Agar (葡萄糖、酵母膏琼脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (琼脂) 15g
pH 7.2
48. MMN Medium (MMN培养基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (柠檬酸) 0.2g
Distilled water (蒸馏水) 900ml pH 5.5 (左右)
适用范围:蜜环菌
49. Starch Agar (淀粉琼脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (琼脂) 20g
Distilled water (蒸馏水) 1000ml
50. 5 Be′Wort Agar (5 Be′麦芽汁琼脂)
适用范围:园弧青霉、少根根霉、日本根霉、爪哇根霉
51. 5 Be′Koji Extract Agar (5 Be′米曲汁琼脂)
适用范围:华根霉、三宝垄根霉
52. 发根农杆菌培养基(YEB)
Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L
Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (琼脂) 1.5g/100ml
pH 7.4
适用范围:青枯假单胞菌
53. 青枯菌培养基 (Tm)
Peptone (蛋白胨) 10g Casein (干酪素水解物产) 1g
Glucose (葡萄糖) 5g Ddhro (纯水) 1000ml
Agar(琼脂) 15g pH 7.0
54. YM (Yeast Malt Agar)
酵母膏 3.0g 麦芽汁 3.0g
蛋白胨 5.0g 琼脂 20.0g
蒸馏水 1000ml
55. ISP 4
可溶性淀粉 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
琼脂 20.0g 蒸馏水 1000ml
56. Trypton Soy Agar
胰蛋白胨 17.0g 大豆胨 3.0g
葡萄糖 2.5g 琼脂 20.0g
NaCl 5.0g K2HPO4 2.5g
蒸馏水 1000ml
57. NA (Nutrient Agar)
牛肉膏 3.0g 蛋白胨 5.0g
琼脂 20.0g 蒸馏水 1000ml
pH 6.6-7.0
58. 甘露(糖)醇琼脂 (ATCC 1)
酵母膏 5.0g 蛋白胨 3.0g
甘露(糖)醇 25.0g 琼脂 15.0g
蒸馏水 1000ml
59. Marine琼脂 2216 (ATCC 2)
蛋白胨 5.0g 酵母膏 1.0g
柠檬酸铁 0.1g 溴化钾 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
氯化锶 0.034g 硼酸 0.022g
硅酸钠 0.004g 氟化钠 0.0024g
琼脂 15.0g 蒸馏水 1000ml
pH 7.4-7.8
60. 兔血琼脂(ATCC 4)
牛心(取其浸液) 500.0g 胰蛋白胨 (Tryptose) 10.0g
NaCl 5.0g 琼脂 15.0g
蒸馏水 1000ml pH 6.6-7.0
61. 孢子形成培养基 (ATCC 5)
酵母膏 1.0g 牛肉膏 1.0g
胰蛋白胨 2.0g 葡萄糖 10.0g
FeSO4 微量 琼脂 15.0g
蒸馏水 1000ml pH 7.2
62. Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g 琼脂 15.0g
蒸馏水 1000ml
63. YPAD (ATCC 109)
酵母膏 10.0g 蛋白胨 20.0g
葡萄糖 20.0g 琼脂 20.0g
蒸馏水 1000ml pH 7.0
64. SP (ATCC 432)
棉子糖 1.0g 蔗糖 1.0g
半乳糖 1g 可溶性淀粉 5g
水解酪蛋白 2.5g 琼脂 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
蒸馏水 1000ml pH 自然
65. 胰酪胨大豆酵母浸膏琼脂 (TSA-YE)
胰蛋白胨 15.5g 大豆蛋白胨 5.0g
NaCl 5.0g 酵母膏 6.5g
琼脂 15.0g 蒸馏水 1000ml
pH 7.3
66. ATCC 20
蛋白胨 5.0g 酵母膏 15.0g
K2HPO4 3.0g 葡萄糖 2.0g
琼脂 20.0g 蒸馏水 1000ml
pH 7.3-7.5