【作者】 饶钦雄； 宣岩芳； 白冰； 董茂锋； 周苏； 邢增涛；
【Author】 RAO Qinxiong,XUAN Yanfang,BAI Bing,DONG Maofeng,ZHOU Su,XING Zengtao Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Supervision and Testing Center for Edible Fungi Quality at Shanghai,Ministry of Agriculture,Shanghai 201403,China
【机构】 上海市农业科学院农产品质量标准与检测技术研究所农业部食用菌产品质量监督检验测试中心(上海)；
【摘要】 建立食用菌中福美双残留量的高效液相色谱(HPLC)检测方法。新鲜食用菌子实体样品粉碎后经二氯甲烷超声(53Hz)提取20min,离心(3320 g、5min),二氯甲烷经氮气吹干,甲醇定容后用HPLC测定。以等体积甲醇和0.1%甲酸水溶液为流动相,1mL/min等度洗脱,在0.05～10μg/mL范围内,福美双的峰面积与其浓度呈线性相关,R≥0.999,方法检出限为0.02mg/kg,添加回收率为79.4%～90.2%,变异系数为1.15%～7.51%%。
【Abstract】 A method for determining thiram residues in fruit bodies of edible fungi based on high performance liquid chromatography(HPLC) is described.Fresh fruit bodies were cut mechanically into small pieces and extracted with dichloromethane for 20 min using ultrasound.After centrifugation(3320 g,5 min),the supernatant was evaporated to dryness under nitrogen,the residue was re-dissolved in methanol,and samples were analyzed by HPLC.Separation was achieved at a flow rate of 1 mL/min using an isocratic e...
【关键词】 福美双； 食用菌； 子实体； 高效液相色谱；
【Key words】 Thiram； edible fungi； fruit bodies； high-performance liquid chromatography；
【基金】 上海科委标准化技术专项(编号:10DZ0503800)的部分研究内容
【分类号】S481.8