不同产地猴头菌子实体粗多糖特征及其刺激巨噬细胞产生NO的活性
Partial Characterization of Polysaccharides from Seven Hericium erinaceus Fruit Body Samples and Their Effect on Nitric Oxide Release from Macrophages
【作者】 吴迪; 张劲松; 蒋俊; 周帅; 汪雯翰; 刘艳芳; 冯娜; 唐庆九; 罗珍; 杨焱;
【Author】 WU Di1,ZHANG Jingsong1,JIANG Jun1,2,ZHOU Shuai1,WANG Wenhan1, LIU Yanfang1,FENG Na1,TANG Qingjiu1,LUO Zhen3,YANG Yan1 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China; 2College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China; 3Infinitus(China) Company Ltd,Guangzhou,Guangdong 510000,China
【机构】 农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所; 南京农业大学生命科学学院; 无限极(中国)有限公司;
【摘要】 提取7种不同来源的猴头菌(Hericium erinaceus)子实体粗多糖,对其理化性质和体外免疫活性进行分析。结果表明:不同产地的猴头菌子实体粗多糖含量和得率差异较大,以来源于黑龙江省牡丹江市海林县(H1)、林口县(H3)的子实体粗多糖含量、得率较高;各粗多糖提取物分子量分布相似,单糖组成均以葡萄糖为主,并含有岩藻糖和半乳糖;7种子实体粗多糖提取物均能增强小鼠巨噬细胞株RAW264.7生成NO的量,以500μg/mL H3提取物活性最高、H1次之。
【Abstract】 Crude polysaccharide preparations were prepared by hot(100 ℃) water extraction and ethanol precipitation from Hericium erinaceus fruit bodies artificially cultivated at seven different facilities located in Fujian,Zhejiang and Heilongjiang Provinces of China.Highest yields of crude extract containing the highest amounts of polysaccharide were obtained from fruit body samples(H1 and H3) grown in Heilongjiang.HPLC analysis revealed that crude polysaccharide material extracted from the seven fruit ...
【关键词】 猴头菌; 粗多糖; 一氧化氮释放; 巨噬细胞; 免疫调节;
【Key words】 Hericium erinaceus; polysaccharide; nitric oxide production; macrophages; immunoregulation;
【基金】 上海市农委重点攻关项目[编号:沪农科攻字(2008)第8-2号]的部分研究内容
【分类号】S646.9