野生硫磺菌分离株ITS序列分析
ITS Sequence Analysis of Strain ts Isolated from a Fruit Body of Laetiporus sulphureus Growing on Tai Mountain
【作者】 兰玉菲； 王庆武； 安秀荣； 王明才；
【Author】 LAN Yufei,WANG Qingwu,AN Xiurong,WANG MingcaiEdible Fungi Research Institute,Tai’an Academy of Agricultural Sciences,Tai’an,Shandong 271000,China
【机构】 泰安市农业科学研究院食用菌研究所；
【摘要】 采用组织分离的方法从野生硫磺菌(Laetiporus sulphureus)子实体上分离得到菌株ts,以其基因组为模板扩增获得该菌株的ITS序列,序列分析结果表明:该菌株与GenBank中Laetiporus sulphureus var.sulphureus的3个日本分离株聚为一簇,ITS核苷酸一致率为98.31%～99.25%,而与其它22个来自不同国家的硫磺菌属菌株的ITS核苷酸一致率为91.93%～98.12%,依此结果,ts菌株应为L.sulphureus var.sulphureus。
【Abstract】 Fungal mycelium,isolated from a fruit body of Laetiporus sulphureus growing in the wild on a species of Quercus located on Tai Mountain,was identified as a strain(designated strain ts) of L.sulphureus var.sulphureus based on ITS sequencing.The ITS sequence(deposited in GenBank under the accession number,JF946748) was used along with ITS sequences from other Laetiporus species to construct a phylogenetic tree using the neighbor-joining method.This revealed a high genetic similarity(98.31%-99.25%)...
【关键词】 硫磺菌硫磺原变种； 转录间隔区；
【Key words】 Laetiporus sulphureus var.sulphureus； internal transcribed spacers(ITS)；
【基金】 山东省中青年科学家科研奖励基金(编号:BS2011NY021);泰安市科技发展计划项目(编号:20113008)的部分研究内容
【分类号】S646;S567.39