一种不经热浴从双孢蘑菇子实体中提取DNA的新方法(英文)
A New Protocol for Extracting DNA from Agaricus bisporus Fruit Bodies without Thermal Incubation
【作者】 姬宏超； 万艳娟； 莫美华； 徐学锋；
【Author】 JI Hongchao,WAN Yanjuan,MO Meihua,XU XuefengCollege of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642,China
【机构】 华南农业大学食品学院；
【摘要】 介绍一种不经热浴快速从双孢蘑菇子实体中提取DNA的新方法,并与传统的CTAB和SDS法相比较,结果显示:该方法得到的DNA完整性好,时间短,步骤少,有毒试剂用量少,在提取DNA的质量、得率、时间及完整性等方面都优于传统的CTAB和SDS法,为食药用菌子实体DNA的提取参考。
【Abstract】 A new,simple and rapid method for obtaining DNA from Agaricus bisporus fruit bodies is described and compared with the widely used CTAB and SDS methods in terms of yield,DNA quality and integrity,and duration of extraction time.Total DNA quality was estimated by restriction endonuclease digestion and by ITS,ISSR and RAPD analyses using standard protocols.Our data demonstrated that the new method renders improved yields of total DNA of high quality and integrity,and is less time-consuming than ex...
【关键词】 双孢蘑菇； DNA提取； 内切酶消解；
【Key words】 Agaricus bisporus； DNA isolation； restriction endonuclease digestion；
【基金】 Supported by National Natural Science Foundation of China(No.30800774)
【分类号】S646