王怡平; 肖亚中; 沈益寿; 武廷章; 沈中兴
安徽大学生物系
【中文摘要】 安徽繁昌泥炭经混合酸水解获得的提取液，１：１稀释后作为食用菌Ｐ，ｄｊｅｍｏｕｒ深层发酵的底物。在没有葡萄糖和其他碳源营养补充时，该泥炭提取液中９５％的总碳水化合物可以被食用菌Ｐ，ｄｊｅｍｏｕｒ利用，并生成球形菌丝休，菌丝干重７克／升。正交试验结果显示：在１：１稀释的泥炭提取液中补加１．５％葡萄糖，０．５％酵母抽提物和０．１％的蛋白胨时，平均菌丝干重为１２．４４克／升。最高菌丝产量１３．１克／升。方差分析结果表明：葡萄糖水平均变化对产量影响最大；酵母抽提物添加量在０．３～０．５％平变化对菌丝产量无显著影响。
【英文摘要】 The production of mushroom mycelian pellets by Pleurotus djamotir from acidic peat extract was tested in shake-flask fermentation. This peat extract was obtained from Fan-Chang peatland near Wuhu, Anhui, China,Without supplement of glucose,95 percent of carbonhydrate from the peat extract diluted in the ratio 1:1 with water was utilized as a carbon nutrient source by P.djamour,The best result of 7g dry myce-lium/L was observed when the peat extract was supplemented only by 0.5%yeast extract. Adding glucose moderately to above culture can enhence the growth of P. djamour. However,adding 2.59% glucose can inhibit the grwoth of mycelium,In order to find out the optimum formula of peat extract complex medium,the orthogonal design with three factors at three levels was applied in shake-flask experiments.The variance analysis of orthogonal test shows that the optimum formula with the average weight of l2.44f dry mycelium/L consists of 0.5%yeast extract,1.5%glucose and 0.1%peptone,and that tl...更多ie influence of the concentration of glucose supplemented on the production of mushr-oom mycelium was extreamly remarkble, while the influence of yeast extract was not impressive at above three levels. 还原
【中文关键词】 泥炭提取液; 深层培养; 正交试验; 食用菌; 菌丝小球; Pleurotusdjamour
【英文关键词】 peat extract; submerged cultivation; mushroom mycelian pellets; orthog-onal design; Pleurotus djamour.
【基金】安徽省科委重点资助
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1995年02期 【DOI】CNKI:SUN:ZSYJ.0.1995-02-015