吴映明; 陈爱葵; 彭锦红; 梁美玲
广东教育学院生物系; 广东教育学院生物系; 广东教育学院生物系; 广东教育学院生物系 广州510303; 广州510303; 广州510303; 广州510303
【中文摘要】 利用苯酚-硫酸法对破壁处理后的灵芝孢子粉多糖含量进行测定,结果表明采用超低温冻融处理和超声波处理进行灵芝孢子粉破壁均具有效果好、成本低及操作简便等特点;增加冻结-解冻循环次数在一定程度上能提高多糖含量,循环次数以5次～7次为宜;将超声波处理法和超低温冻融法很好地结合起来,其多糖释放更高,效果更佳,此方法更具实用价值。
【英文摘要】 The measurement of polysaccharides in sporoderm-broken spore was analysed by the method of phenol-sulfuric acid. The result shows that it is an effective, economic and facile way to break the cellular wall with thawing and freezing of ultra-low temperture or ultrasonic. And the circulating times of thawing and freezing can increase the contents of polysaccharides in a certain degree and the sug-gested number is 5～7. The method of combining ultrasonic with thawing and freezing of ultra-low temperture commendably can release the polysaccharides of its contents higher and be of more practical value.
【中文关键词】 灵芝孢子粉; 灵芝多糖; 超低温冻融; 超声波
【英文关键词】 Ganoderma lucidum spore powder; Polysaccharides in Ganoderma lucidum; Thawing and freezing of ultra-low temperture; Ultrasonic
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2008年03期 【DOI】CNKI:SUN:ZSYJ.0.2008-03-016