黄春燕; 单洪涛; 刘传富; 皇传华; 万鲁长; 郭惠东; 杨化恩; 张福进
山东省农科院植保所; 山东省农科院植保所; 济南市农科所; 济南市农科所; 山东省农科院植保所; 山东省农科院植保所; 临沂市河东区农业局; 威海市环翠区植保站 济南250100 ; 济南250100 ; 济南250023 ; 济南250023 ; 济南250100 ; 济南250100 ; 临沂276000 ; 威海264000
【中文摘要】 研究了糙皮侧耳液体菌种适宜的培养条件、培养周期与贮存期。结果表明,糙皮侧耳液体菌种适宜的培养基配方为蔗糖2%,玉米粉4%,麦麸3%,酵母粉0.3%,KH2PO40.25%,Mg SO4·7H2O0.1%,ZnSO40.05%,pH6.17。摇瓶培养装液量80mL/250mL,摇床转速150r/min,接种量10%,培养周期6d,菌种于3d内用完为宜。
【英文摘要】 The suitable culture conditions, the culture period and the preservation time of the liquid spawn of P. ostreatus were studied. The experiment results showed that the optimum formula for P. ostreatus liquid spawn medium was as follows: sucrose 2%, corn meal 4%, wheat bran 3%, yeast powder 0.3%, KH_2PO_4 0.25%, MgSO_4·7H_2O 0.1%, ZnSO_4 0.05%, and the optimum pH was 6.17. While the optimum medium capacity, the rotation rate, the spawning rate and the culture period were 80mL/250mL, 150r/min,10% and 6d resp.. And it would be better to use the spawn within 3 days.
【中文关键词】 糙皮侧耳; 液体菌种; 培养条件; 培养周期; 贮存期
【英文关键词】 Pleurotus ostreatus; Liquid spawn; Culture condition; Culture period; Preservation time
【基金】山东省济南市科技局科技攻关项目"食用菌菌种引进选育及研究"[济科合字(2001)第70-1号]的部分内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-009