李向敏; 谢意珍; 潘鸿辉; 简伟明; 罗彪; 李森柱; 张智
广东粤微食用菌技术有限公司; 广东省微生物研究所
【中文摘要】 利用多种分析检测手段和肿瘤细胞实验,研究了灵芝孢子油的质量检测参数与其抗肿瘤细胞活性的关系,为灵芝孢子油质量检测标准的建立提供实验参考依据。取6种不同的经CO2超临界提取的灵芝孢子油样品,用KOH标准滴定法测酸价、KI-I2法检测过氧化值、紫外-可见光分光光度法测总三萜。同时利用肿瘤细胞检测6种样品对人恶性乳腺癌细胞(MT-1)和淋巴瘤细胞(Jurka)t的抑制作用。结果表明,样品的酸价与总三萜含量和其抗肿瘤效果呈正相关,6种样品的过氧化值变化不大。高酸价样品1,其总三萜含量(16.00%)较高,在1μL·mL-1浓度抑制时,能够抑制绝大部分肿瘤细胞的生长,具有较高的抗肿瘤活性。
【英文摘要】 The relationship of Ganoderma lucidum spore oil quality testing standards and its anti-tumor cell activity to provide parameters for the establishment of testing standards were studied. Ganoderma lucidum spore oil was extracted by CO2 supercritical fluid, which had six different samples. The acid value, the peroxide value, total triterpenoids were determined. The inhibition effect of Ganoderma lucidum spore oil on human malignant breast cancer cells (MT-1) and human leukemia cells (Ju-rkat) were studied by cell culture method. The results showed that anti-tumor effect was positively correlated with acid value and the total level of triterpenoids.
【中文关键词】 灵芝孢子油; 三萜; 抗肿瘤细胞活性
【英文关键词】 Ganoderma lucidum spore oil; Total triterpenoids; Anti-tumor cell activity
【基金】广州开发区经济区科技资助项目(2009Q-P143);; 广东省科技厅农业攻关资助项目(2009B080701065)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-019